Summary
In comparison to values obtained in normal-weight normolipenic controls, dilute blood
clot lysis time was found to be prolonged in resting and exercised patients with endogenous
hypertriglyceridemia. Lysis time was also prolonged in overweight subjects with only
slightly increased serum lipids but it was not significantly changed in type II-a
hyperlipoproteinemia. An obvious acceleration of clot lysis was noted in patients
with decompensated cirrhosis of the liver. Inclusion of p-chlormercuribenzoate (PCMB)
into the diluted blood clot caused an acceleration of lysis time. This effect was
particularly obvious with hypertriglyceridemic blood and minimal with blood obtained
from cirrhotic patients. Addition of various amounts of heat-defibrinated plasma to
blood from cirrhotic patients caused a proportional prolongation of clot lysis time
and this effect was greatly diminished in the presence of PCMB. Similar amounts of
serum had a much lesser effect than defibrinated plasma. Inhibition of fibrin cross-linking
by PCMB was confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis.
The present data complete previous observations concerning a higher plasma factor
XIII activity in type II-b and type IV hyperlipoproteinemia and suggest that an increased
crosslinking of fibrin might partially explain the deficient thrombolysis of hypertriglyceridemic
patients.
